Genetic Analysis and Epitope Prediction of SARS-CoV-2 Genome in Bahia, Brazil: An In Silico Analysis of First and Second Wave Genomics Diversity

COVID-19 is an infectious disease caused by SARS-CoV-2. This virus presents high levels of mutation and transmissibility, which contributed to the emergence of the pandemic. Our study aimed to analyze, in silico, the genomic diversity of SARS-CoV-2 strains in Bahia State by comparing patterns in variability of strains circulating in Brazil with the first isolated strain NC_045512 (reference sequence). Genomes were collected using GISAID, and subsequently aligned and compared using structural and functional genomic annotation. A total of 744 genomes were selected, and 20,773 mutations were found, most of which were of the SNP type. Most of the samples presented low mutational impact, and of the samples, the P.1 (360) lineage possessed the highest prevalence. The most prevalent epitopes were associated with the ORF1ab protein, and in addition to P.1, twenty-one other lineages were also detected during the study period, notably B.1.1.33 (78). The phylogenetic tree revealed that SARS-CoV-2 variants isolated from Bahia were clustered closely together. It is expected that the data collected will help provide a better epidemiological understanding of the COVID-19 pandemic (especially in Bahia), as well as helping to develop more effective vaccines that allow less immunogenic escape. [ FROM AUTHOR] Copyright of COVID is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)

The Potential of Vouacapanes from Pterodon emarginatus Vogel Against COVID-19 Cytokine Storm.

Purpose: The emergence of the COVID-19 pandemic has led to the search for potential therapeutic responses for various aspects of this disease. Fruits of Pterodon emarginatus Vogel (Fabaceae), sucupira, have been used in Brazilian traditional medicine because of their anti-inflammatory properties, which have been proven in vivo, in vitro, and in silico. Therefore, the aim of this work is to evaluate P. emarginatus oleoresin and isolated diterpenes by in vitro anti-inflammatory models. Methods: In this study, the mechanisms underlying the anti-inflammatory activity of P. emarginatus oleoresin and vouacapanes 6α,19ß-diacetoxy-7ß,14ß-dihydroxyvouacapan (V1), 6α-acetoxy-7ß,14ß-dihydroxyvouacapan (V2), and methyl 6α-acetoxy-7ß-hydroxyvouacapan-17ß-oate (V3) were investigated in HaCaT cells. Results: Oleoresin, V2, and V3 inhibited phospholipase A2 (30.78%, 24.96%, and 77.64%, respectively). Both vouacapanes also inhibited the expression of COX-2 (28.3% and 33.17%, respectively). The production of interleukin 6 (IL-6) was inhibited by oleoresin by 35.47%. However, oleoresin did not interfere with Nrf-2 expression or IL-8 production. Conclusion: The results support the ethnomedicinal use of P. emarginatus oleoresin as an anti-inflammatory herbal medicine, and also highlight P. emarginatus oleoresin and isolated vouacapanes as an attractive therapeutic approach for COVID-19 through the reduction or chronological control of the inflammatory mediators IL-6, cyclooxygenase-2 (COX-2), phospholipase A2, and INF-y (indirectly) during the SARS-CoV-2 infection process.

Covid-19 and work dimensions: a socio-historical analysis of impacts affecting men during the pandemic / Covid-19 e as dimensões do trabalho: análise sócio-histórica dos impactos vivenciados por homens na pandemia / COVID-19 y las dimensiones del trabajo: análisis sociohistórico de los impactos experimentados por los hombres en la pandemia

OBJETIVO: Apreender os impactos vivenciados por homens residentes no Brasil na pandemia da Covid-19 em relação às dimensões do trabalho. MÉTODO: Estudo sócio-histórico, qualitativo, realizado durante a pandemia da Covid-19 com 400 adultos e idosos residentes no Brasil. Aplicou-se um formulário on-line. Os dados foram analisados com base no Discurso do Sujeito Coletivo, interpretados à luz do Materialismo Histórico Dialético. RESULTADOS: A pandemia da Covid-19 provocou e intensificou impactos: sobrecarga provocada pelo trabalho home office; inadequações no trabalho e exposição à contaminação pelo Coronavírus; estresse e medo de ser contaminado no trabalho e de perder o emprego; dificuldade na manutenção econômica/financeira e adaptações repentinas no desempenho do trabalho. CONCLUSÃO: As dimensões do mundo do trabalho intensificaram as vulnerabilidades sociais e em saúde de homens no contexto da pandemia da Covid-19. Repercutiram em maior exposição ao SARS-CoV-2 e vivência de estressores na vida cotidiana.

OBJECTIVE: To apprehend the impacts affecting men residing in Brazil during the Covid-19 pandemic concerning work dimensions. METHOD: A socio-historical, qualitative study carried out during the Covid-19 pandemic with 400 adult and old males residing in Brazil. An on-line form was applied. Data were analyzed based on the Discourse of the Collective Subject, interpreted according to the Dialectic Historical Materialism. RESULTS: The Covid-19 pandemic did cause and intensify impacts: overload resulting from home-office jobs; inadequacies as to the work and exposition to contamination by the Coronavirus; stress and fear to be contaminated at work and losing employment; difficulties to maintain economic/financial conditions and unexpected adaptations in work performance. CONCLUSION: The dimensions of the work environment did intensify both social and health vulnerabilities for men in the context of the Covid-19 pandemic, rebounding on even higher exposure to SARS-CoV 2 and daily life stressors.

OBJETIVO: Comprender los impactos experimentados por los hombres residentes en Brasil en la pandemia de COVID-19 respecto a las dimensiones del trabajo. MÉTODO: Estudio sociohistórico, cualitativo, realizado durante la pandemia de COVID-19, con 400 adultos y personas mayores residentes en Brasil. Se aplicó un formulario online. Los datos se analizaron con base en el Discurso del Sujeto Colectivo y fueron interpretados a la luz del Materialismo Histórico Dialéctico. RESULTADOS: La pandemia de COVID-19 ha ocasionado e intensificado los impactos: sobrecarga causada por el teletrabajo; inadecuaciones en el trabajo y exposición a la contaminación por el coronavirus; estrés y miedo a contaminarse en el trabajo y a perder el empleo; dificultad en el mantenimiento económico/financiero y adaptaciones repentinas en el rendimiento del trabajo. CONCLUSIÓN: Las dimensiones del mundo del trabajo han intensificado las vulnerabilidades sociales y de salud de los hombres en el contexto de la pandemia de COVID-19. Han repercutido en una mayor exposición al SARS-CoV-2 y vivencia de estresores en la vida cotidiana.

Effect of immunonutrition on serum levels of C-reactive protein and lymphocytes in patients with COVID-19: a randomized, controlled, double-blind clinical trial.

INTRODUCTION: Introduction: patients with COVID-19 undergo changes in leukocyte count, respiratory disorders, and an increase in inflammatory substances. To improve the inflammatory condition, some nutrients can be used, including arginine, omega-3 fatty acids and nucleotides. This study aims to evaluate how oral immunonutrient supplements affects serum C-reactive protein (CRP) levels and lymphocyte count in patients with COVID-19. Methods: in this double-blind clinical trial, we randomized 43 adult patients with COVID-19 to receive a standard high-protein normocaloric supplement (control) or an immunonutrient-enriched supplement (experiment) for 7 days. The primary outcome was to evaluate changes in total lymphocyte count and serum level of CRP. The assessment of risk and nutritional status of these patients was also performed. Results: forty-three patients with mean age of 41.5 (± 1.8) years were followed up, 39.5 % of them women. The mean body mass index was 27.6 (± 0.8) kg/m² and 58.1 % had low nutritional risk. In the experiment group, there was a CRP reduction of 23.6 (± 7.5) mg/L, while in the control branch the decrease was 14.8 (± 12.1) mg/L (p = 0.002). There was an increase in lymphocytes in the experiment group (+367.5 ± 401.8 cells/mm³) and a reduction in the control group (-282.8 ± 327.8 cells/mm³), although there was no statistical significance (p = 0.369). Relative risk (RR) of treatment in reducing CRP by 30 % or more was 4.45 (p < 0.001; 95 % CI, 1.79-11.07). RR in increasing lymphocyte count by 30 % or more was 1.28 (p = 0.327; 95 % CI, 0.67-2.45). Conclusion: we conclude that immunonutrient supplements seem to reduce CRP levels more than standard high-protein normocaloric supplements.

INTRODUCCIÓN: Introducción: los pacientes con COVID-19 sufren cambios en el recuento de leucocitos, trastornos respiratorios y aumento de sustancias inflamatorias. Para mejorar la condición inflamatoria se pueden usar algunos nutrientes, como la arginina, los ácidos grasos omega-3 y los nucleótidos. Este estudio tiene como objetivo evaluar cómo los suplementos de inmunonutrientes orales afectan a los niveles séricos de proteína C-reactiva (PCR) y al recuento de linfocitos en pacientes con COVID-19. Métodos: en este ensayo clínico doble ciego, aleatorizamos a 43 pacientes adultos con COVID-19 para recibir un suplemento normocalórico estándar alto en proteínas (control) o un suplemento enriquecido con inmunonutrientes (experimento) durante 7 días. El resultado primario fue evaluar los cambios en el recuento total de linfocitos y el nivel sérico de PCR. También se realizó la evaluación del riesgo y el estado nutricional de estos pacientes. Resultados: cuarenta y tres pacientes con edad media de 41,5 (± 1,8) años fueron seguidos, el 39,5 % de ellos mujeres. El índice de masa corporal medio fue de 27,6 (± 0,8) kg/m² y el 58,1 % tenían bajo riesgo nutricional. En el grupo experimental hubo una reducción de la PCR de 23,6 (± 7,5) mg/L, mientras que en la rama de control la disminución fue de 14,8 (± 12,1) mg/L (p = 0,002). Hubo un aumento de linfocitos en el grupo experimental (+367,5 ± 401,8 células/mm³) y una reducción en el grupo de control (-282,8 ± 327,8 células/mm³), aunque no hubo significación estadística (p = 0,369). El riesgo relativo (RR) del tratamiento para reducir la PCR en un 30 % o más fue de 4,45 (p < 0,001; IC 95 %: 1,79-11,07). El RR en el aumento del recuento de linfocitos en un 30 % o más fue de 1,28 (p = 0,327; IC 95 %: 0,67-2,45). Conclusión: se concluye que los suplementos de inmunonutrientes parecen reducir los niveles de PCR más que los suplementos normocalóricos estándar altos en proteína.